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Hi everyone,
Sorry for an off-topic question:
I have a protein which only binds its positive charged ligand at pH
above 6, unfortunately it can only be crystallised at pH4. I saw nothing
in the pocket finally. The reason could be that a carboxylate essential
to the binding are protonated at low pH. I thought the mutagenesis on
the molecular surface could be a solution. Does anyone have such
experience? Could you suggest the mutagenesis strategy or anything else
to increase the crystallisation pH?
Thank you very much in advance.
Best regards
Lei
