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Hi Orly, it looks like your problem may lie at the protein level, so I fear that you may need a new construct(s) in the very near future... Nonetheless, I would try first some limited proteolysis experiments on your purified protein to see if you can 'trim' your protein just enough to produce a more ordered molecule. You can then try to crystallize with that version of the protein. Actually such experiments can even tell you previously unanticipated things about the domain organization of your protein, so they are worth the couple of days that you will spend on them. Good luck Savvas Ghent University, Belgium Quoting Orly Dym <[EMAIL PROTECTED]>: > > I have beautiful reasonably big crystals from a protein which > diffract to 6A at the most. > I have tried adding additives, crystallizing in different methods > (hanging, drops, sitting drops and microbatch under oil). > The crystals grow over night and contain high percent of water (they > are very fragile). > I also tried different methods of dehydration (using different PEG's, > MPD, glycerol and so on). > Currently I am trying to grow them at 4C. > > Any other ideas as to what I should try in order to improve the > resolution? > > Thanks > > Orly > > Dr. Orly Dym > Israel Structural Proteomics Center > Department of Structural Biology > Weizmann Institute of Science > http://www.weizmann.ac.il/ISPC > 972-8-934-3759 - Tel > 972-8-934-4159 - Fax > [EMAIL PROTECTED] > > > > --
