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Dear Tiago
Raman Optical Activity (ROA) is a new spectroscopic technique that
gives information about secondary structures and folds.
See a recent paper by Zhu et al (Structure vol 13 1409-1419, 2005)
for a review of the technique.
Regards
neil
On 12 Dec 2005, at 16:37, Tiago Barros wrote:
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Dear all,
Apologies for a non-ccp4 subject.
I’ve been assigned to give a small talk in our “X-ray meeting” here
at the lab. I thought it would be interesting to discuss how to
analyse protein crystals without X-rays. Obviously our ultimate
goal is a diffraction pattern good enough to solve our structure,
but in many cases other valuable information could be obtained
using other, complementary techniques.
Working with a colourful protein, multiple spectroscopic methods
(absorption, fluorescence, etc) popped up in my mind as examples of
those techniques. Chromatographic methods (HPLC?) for chemical
analysis also look likely candidates. What else could you add to
the discussion?
Does anyone have personal experience on “How to analyse crystals
without X-rays”?
Thank you very much for any suggestions.
Tiago
------------------------------------------------------------
Tiago Barros
Max-Planck Institute of Biophysics
Dept. of Structural Biology
Max-von-Laue Str. 3
D-60438 Frankfurt
Germany
tel: +49-69-6303-3055
Neil Isaacs
Department of Chemistry
University of Glasgow
Glasgow G12 8QQ
Tel: +44 (0)141 330 5954
Fax: +44 (0)141 330 4888
[EMAIL PROTECTED]
