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See
"Structure of rat BCKD kinase: Nucleotide-induced domain communication
in a mitochondrial protein kinase"
Mischa Machius, Jacinta L. Chuang, R. Max Wynn, Diana R. Tomchick, and
David T. Chuang, PNAS vol. 98, pp. 11218-11223 (2001).
To purify, concentrate and crystallize the enzyme, buffer that
contained 10% glycerol, 1 M NaCl, 250 mM KCl and 300 mM arginine was
used. The ratio of salts and glycerol used was optimized for best
crystal growth; concentration of the enzyme to 20 mg/ml was impossible
without this buffer.
Diana
On Jan 24, 2006, at 10:18 AM, Stein, ND ((Norman)) wrote:
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Hi all,
I was wondering if anyone's had any experience using arginine as a
crystallization additive, and generally as a useful chemical to prevent
aggregation of globular, but sticky proteins. The paper that set us
thinking
about is: Journal of Chromatography A, 1094 (2005) 49 - 55.
Thanks in advance for any tip and advice,
Luca
#########################################
Luca Pellegrini
80 Tennis Court Road
Biochemistry Department
University of Cambridge
Cambridge CB2 1GA - UK
phone:00441223333667 (office)
333662 (lab)
fax: 00441223766002
email: [EMAIL PROTECTED]
### a low-throughput crystallographer ###
* * * * * * * * * * * * * * * * * * * * * * * * * * * *
Diana R. Tomchick
Assistant Professor
University of Texas Southwestern Medical Center
Department of Biochemistry
5323 Harry Hines Blvd.
Rm. ND10.214B
Dallas, TX 75390-8816, U.S.A.
Email: [EMAIL PROTECTED]
214-645-6383 (phone)
214-645-6353 (fax)
