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Dear all, Arginine had no positive effect on crystallization success when we tried it on 13 commercially available proteins in Al's oil microbatch crystallization experiments. But we only tested Arg as coprecipitant/additive with oxalate and benzoate. Mixtures with arginine crystallized less proteins (5,3) than oxalate or benzoate alone (10,4 respectively). It is impotant to note that the proteins used in the study were predominantly stable and well behaving and that we did not test Arg as additive to a standard crystallization screen or to problematic proteins. We also tried Arginine as the only precipitant at starting concentrations ranging from 0.05 to 0.2M. Arginine crystallized the smallest number of proteins (4) of all the acids/salts tested (oxalate, malonate, citric acid, succinate, formate, benzoate, glutamate). There is no publication, my apologies. This precipitant study with organic acids was presented as a poster at ICCBM 2002, though. Best regards, Armin -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Charles W. Carter Jr. Sent: Tuesday, January 24, 2006 20:31 To: ccp4bb Subject: Fwd: [ccp4bb]: arginine as crystallization additive *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** >> I'm grateful to Dan for this citation! I thought of replying to the >> initial request. >> >> I think that there is a reference in this chapter to a paper where I >> got the idea, perhaps from Timasheff. >> >> I have to add that the idea captivated me, together with the notion >> that other additives could serve specific kinds of purposes for >> different protein crystallization problems. Unfortunately, despite >> some considerable effort to demonstrate an effect (either positive or >> negative) of arginine on several systems I was interested in >> crystallizing came to nought. I do recollect that a crystal structure >> of Grb2 from Arnaud Ducruix's lab may have benefitted from the use of >> arginine in the crystallization mix. However, that example was >> anecdotal and did not venture into the arena of experimental proof. >> Thus, arginine joins an (impossibly) long list of possibilities for >> which there are arguments, but little data. >> >> On Jan 24, 2006, at 1:07 PM, Daniel Anderson wrote: >> >>> *** For details on how to be removed from this list visit the *** >>> *** CCP4 home page http://www.ccp4.ac.uk *** >>> >>> >>> >>> Yes, there's a long-ish history of using arginine as crystallization >>> additive. See for examples: >>> >>> Charlie Carter's Chapter 4 (pages 75-120) in "Crystallization of >>> Nucleic Acids and Proteins", second edition, Ducruix, Giege, >>> editors. >>> >>> DeLucas, et al J.Struct. Biol. (2003) 142, 188-206. >>> >>> There are likely others. >>> >>> -Dan Anderson >>> >>> On Tue, 24 Jan 2006, Stein, ND (Norman) wrote: >>> >>>> *** For details on how to be removed from this list visit the *** >>>> *** CCP4 home page http://www.ccp4.ac.uk *** >>>> >>>> >>>> Hi all, >>>> >>>> I was wondering if anyone's had any experience using arginine as a >>>> crystallization additive, and generally as a useful chemical to >>>> prevent aggregation of globular, but sticky proteins. The paper >>>> that set us thinking >>>> about is: Journal of Chromatography A, 1094 (2005) 49 - 55. >>>> >>>> Thanks in advance for any tip and advice, >>>> >>>> Luca >>>> >>>> ######################################### >>>> Luca Pellegrini >>>> 80 Tennis Court Road >>>> Biochemistry Department >>>> University of Cambridge >>>> Cambridge CB2 1GA - UK >>>> phone:00441223333667 (office) >>>> 333662 (lab) >>>> fax: 00441223766002 >>>> email: [EMAIL PROTECTED] >>>> ### a low-throughput crystallographer ### >>>> >>>> >>>> >>> >>> >> **********UNCrystallographers NOTE new website url****************** >> [EMAIL PROTECTED] http://xtal.med.unc.edu/CARTER/Welcome.html >> Department of Biochemistry and Biophysics CB 7260 >> UNC Chapel Hill, Chapel Hill, NC 27599-7260 >> Tel: 919 966-3263 >> FAX 919 966-2852 >> > > **********UNCrystallographers NOTE new website url****************** [EMAIL PROTECTED] http://xtal.med.unc.edu/CARTER/Welcome.html Department of Biochemistry and Biophysics CB 7260 UNC Chapel Hill, Chapel Hill, NC 27599-7260 Tel: 919 966-3263 FAX 919 966-2852
