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Or in Lec1 Chinese Hamster ovary cells which were the original GnTI
knocked out cells.
On Thu, 2 Feb 2006, Anastassis Perrakis wrote:
You can express it in GnTI(i) HEK293S cells.
See
Structure and function in rhodopsin: high-level expression of rhodopsin with
restricted and homogeneous N-glycosylation by a tetracycline-inducible
N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell
line.
GnTI(-) HEK293S
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12370423&query_hl=7&itool=pubmed_docsum
A.
On Feb 2, 2006, at 13:58, Paul McEwan wrote:
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I'm currently working with a glycoprotein and am having great difficulty
deglycosylating it for crystallisation. I can completely remove the sugars
very easily when the protein is denatured (not much good when you're
wanting to crystalise it), but seems completely resistant to treatment with
endo H or pngase F when done under native conditions. Any hints on getting
this to work would be greatly appreciated.
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Dr Paul A. McEwan
Protein Crystallography Group
Centre for Biomolecular Science
Clifton Boulevard
University of Nottingham
Nottingham
NG7 2RD
Tel: 0115 8468009
http://www.nottingham.ac.uk/~pazxtal
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Kelvin Luther
Program in Molecular and Cellular Biology
Stony Brook University
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