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ZhiYi Wei wrote:
*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Sorry for a non-ccp4 question. I am going to learn the theory and technique of electron microscopy and biological samples preparation for cryoEM to determine 3D structures. And I am wandering which book(s) is best for me, a crystallographer. Any suggestions are welcome.
Here's a reply from a friend in the field: Cryotechniques in Biological Electron Microscopy, Eds. R. A. Steinbrecht and K. Zierold. Springer-Verlag: Heidelberg, Berlin, West Germany; New York, New York, USA. Good background about cryo-preservation of biological specimen in general - but not specifically for single particle reconstruction. I have't read the next one so far, but it's more modern and from one of the best experts in single particle cryo-EM: Three-Dimensional Electron Microscopy of Macromolecular Assemblies : Visualization of Biological Molecules in Their Native State, by Frank, Joachim, Oxford University Press, USA And then there are reviews, like: 1: Jiang W, Ludtke SJ. Electron cryomicroscopy of single particles at subnanometer resolution. Curr Opin Struct Biol. 2005 Oct;15(5):571-7. Review. PMID: 16140524 [PubMed - in process] 2: Thuman-Commike PA. Single particle macromolecular structure determination via electron microscopy. FEBS Lett. 2001 Sep 14;505(2):199-205. Review. PMID: 11566176 [PubMed - indexed for MEDLINE] 3: Ruprecht J, Nield J. Determining the structure of biological macromolecules by transmission electron microscopy, single particle analysis and 3D reconstruction. Prog Biophys Mol Biol. 2001;75(3):121-64. Review. PMID: 11376797 [PubMed - indexed for MEDLINE] -- Jürgen Bosch Howard Hughes Medical Institute and University of Washington Dept. of Biochemistry, K-418 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4542 FAX: +1-206-685-7002
