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I would not have any concerns about that. Your drops will likely
transfer less than 1.5 ul of water to the reservoir well, leading to
less than 2% dilution. A lot less than many other variance that are
introduced along the way. Shorter drop to reservoir solution distance,
less reservoir solution surface area etc can all have some effects
relative to the old 24-well hanging drop but in our experience those
effects are minor and there is no reason to assume that the old way is
always better.
Bart
Brian Mark wrote:
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This is a non-CCP4 related question, but one that perhaps other CCP4
users might be concerned with as well.
We've just switched to 96-well plates to set up our crystallization
screens, using 8-channel pipettes to array out the mother liquor and
set up the drops. We can go down to about 1.5 ul protein/1.5 ul mother
liquor drops with accuracy, and currently use 100 ul of mother
liquor/well. This gives only ~33 times the volume of mother liquor to
drop volume per well when initially set up. This ratio seems small
compared to 24-well plates where it's easy to ensure a much larger
excess of mother liquor.
Although a 33 fold excess seems like it should be enough for
sufficient vapour diffusion to occur, does anyone who has experience
with 96 well plates say otherwise??
Thanks in advance,
Brian
-------------------------------------------------------
Brian L. Mark, MSc, PhD
Department of Microbiology
Room 418, Buller Building
University of Manitoba
Winnipeg, Manitoba
CANADA R3T 2N2
Phone (204) 480-1430
Fax (204) 474-7603
