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Hi, have you tried crystal dehydration? Take a look at: Heras B, Martin JL. Post-crystallization treatments for improving diffraction quality of protein crystals. Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80. Epub 2005 Aug 16. Review. Good luck Jose Quoting Rongjin Guan <[EMAIL PROTECTED]>: > Dear All, > > We have a protein that can be esaily crystallized, but diffracts very weak. > We tried many mutations/diffrent constructs, the problem is still there. > > Crystals are very beautiful, big, and appear in many conditions with the > same > shape (same space group, too). None of them diffract better than 4 A. > > I would appreciate if some one can provide some "fresh" ideas. We have tried > many things, before crystallization, after crystallization and during data > collection, etc. > Years passed, no impovement. Very frustrating. > Any ideas will be welcome. > > Thanks > > Rongjin Guan >
