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Hello Jerry, you may want to try crosslinking prior to purification of the complex. Greetings Oliver --------------------- Dr. Oliver H. Weiergraeber IBI-2 (Structural Biology) Research Centre Juelich D-52425 Juelich Germany Phone: +49-2461-612028 Fax: +49-2461-612020 --------------------- ----- Original Message ----- From: Jerry McCully <[EMAIL PROTECTED]> Date: Wednesday, April 19, 2006 2:31 am Subject: [ccp4bb]: the crystallization of low affinity protein-protein complex > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Dear ccp4bb experts: > > > I'm crystallizing a dynamic protein-protein complex. The two > components bind > to each other > > with a micro-molar affinity(This affinity was shown by Biacore3000 > analysis, > but I can not purify the complex by gel-filtration). > > > > > I can get their crystals, respectively. But so far I have not > > got any crystals of the complex even if I tried several molar > ratios between > them. > > > > When I used the complex sample for crystallization, the smaller > component > was easily grown from the conditions containing PEG3350, and the > other from > MPD. > > > > Does anyone have the experience to crystallize this kind of low- > affinity > protein complexes? > > > > Any suggestion will be highly appreciated. > > > > Jerry McCully > > _________________________________________________________________ > Is your PC infected? Get a FREE online computer virus scan from > McAfee® > Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 > >
