*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Hi Dario,
What about: your MPD site is partly occupied by an MPD molecule and
its C2 position by some more electron-rich ion?
Regards,
R.
On 20 Apr 2006, at 16:23, Segura Pena, Dario wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Dear all,
Recently I obtained protein crystal that grew in 60 % MPD. The crystal
diffracts very well to 1.5 Ǻ. It seems that an MPD molecule is
in the
active center of the enzyme, however the atom C2 of the MPD
molecule has a
B factor of 2 after several cycles of refinement with Refmac.
Does anybody have any idea why the there is such a low B factor for
this
atom?.The electron density for the region corresponding to the atom
C2 of
the MPD molecule is visible at 5 sigma level in a 2fo-fc map while
at 3
sigma level there is not electron density for the rest of the
molecule.
I will appreciate any ideas or suggestions.
Sincerely,
Dario
---
Dr. Roberto Steiner
Randall Division of Cell and Molecular Biophysics
New Hunt's House
King's College London
Guy's Campus
London, SE1 1UL
Phone +44 (0)20-7848-8216
Fax +44 (0)20-7848-6435
e-mail [EMAIL PROTECTED]
