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Hello,
I have been working for some time on finding a molecular replacement solution
for an IGG receptor protein with little to show for it. My search model is a
previously published homologue of the protein that my colleagues and I now
suspect differs greatly with our crystallized protein about a central hinge
angle, despite 97% sequence homology between the two. Any attempts at molecular
replacement with the two domains treated separately result in a pdb with the
terminal ends pushed together instead of the ends that where severed about the
hinge region. This is probably due to the fact that the crystal is very tightly
packed and in high symmetry (P 43 21 2).
I was wondering if anyone knows of a way to perform molecular replacement
about a specific hinge region, I have heard rumors of a program that exists for
this very reason but have not been able to find it.
Thanks,
patrick
--
Patrick G. Shaw
Orville Lab
Georgia Institute of Technology
Institute for Bioengineering and Bioscience
315 Ferst Drive, Mailbox #8
Atlanta, GA 30332-0363
Tel: (404)434-2001 (Cell)
(404)894-4075 (Lab)
email: [EMAIL PROTECTED]
