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Patrick, The Patterson Correlation feature in CNS is meant for this type of problem. I have two domains which only rotate about 10 degrees with respect to one another, and it gave me the best answer. Go look in the MR scripts under the Translation Function. PC refinement is done just before the translation search. Cheers, Chris > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Hello, > I have been working for some time on finding a molecular replacement > solution > for an IGG receptor protein with little to show for it. My search model > is a > previously published homologue of the protein that my colleagues and I now > suspect differs greatly with our crystallized protein about a central > hinge > angle, despite 97% sequence homology between the two. Any attempts at > molecular > replacement with the two domains treated separately result in a pdb with > the > terminal ends pushed together instead of the ends that where severed about > the > hinge region. This is probably due to the fact that the crystal is very > tightly > packed and in high symmetry (P 43 21 2). > > I was wondering if anyone knows of a way to perform molecular > replacement > about a specific hinge region, I have heard rumors of a program that > exists for > this very reason but have not been able to find it. > > Thanks, > patrick > > -- > Patrick G. Shaw > Orville Lab > Georgia Institute of Technology > Institute for Bioengineering and Bioscience > 315 Ferst Drive, Mailbox #8 > Atlanta, GA 30332-0363 > Tel: (404)434-2001 (Cell) > (404)894-4075 (Lab) > email: [EMAIL PROTECTED] >
