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This pattern is consistent with a lipidic cubic phase, a lipidic
lamellar phase or other micro-stacks of hydrated bilayers. But, anyway,
"lipid crystals".
Was this a membrane protein?
I have seen several patterns now like this, so I thought this would be a
good opportunity to follow it up. I bounced this off my colleague Bob
Glaeser (who has much more experience than I with lipids, membranes and
the proteins that can't live without them). Bob may chime in if I get
something wrong, but it sounds to me like the most likely explanation
for this diffraction pattern is that you have a lipidic cubic phase
(which would require a fusogenic lipid and also have a ring at 150A) or
a stack of ordered bilayers (concentric spheres or concentric
cyllinders) which you could call a "lamellar phase". The latter could
be expected to form under relatively harsh dehydrating conditions, like
one might find in a crystallization screen.
This probably isn't what you wanted to hear, but I hope it is useful to
you in the long run.
-James Holton
MAD Scientist
Guiqing Huang wrote:
I present a diffraction pattern here in this email as JPEG. The
diffraction area ranges from about 47-27 A as a continuous thick ring
around the beam stop. Does anyone know what kind of material could
generate this diffraction?
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