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Hi All: I have two questions for the bulk solvent model: 1. Maybe I missed this in reading the manual, but in Refmac is there a way of inputting an mask to define the bulk solvent? Or it is always done automatically using the keyword SOLVENT? 2. Is the bulk solvent model in Refmac somehow different from that in CNS? For one of my low resolution data to 7A, the bulk solvent model in Refmac seems to behave much better than that in CNS. I only use rigid body refinement and there is no TLS refinement in Refmac. So in this case, maybe the major difference of R factor using these two programs would come from the bulk solvent models (maybe also the scaling of Fc to Fobs, or maximum likelyhood, etc.?). Anyway the R factors differ by 10% using the two different programs (40% in CNS vs. 30% in Refmac) when both have a bulk solvent model. If I turn off bulk solvent estimation in CNS, the R factor drops by 5% (i.e. now R=35% in CNS). Regards, Weikai
