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On Jun 8 2006, [EMAIL PROTECTED] wrote:
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Dear All:
I know it is possible to use EM map for model of molecular replacement.
I wonder if anyone can tell me how exactly to do it. I have a 10 A EM map
and 3 A X-ray data. I can convert EM map (ccp4 format) into mtz file by
using mapman and sFALL. Which software I should use to do molecular
replacement and how to set its parameters. After getting the solution
from the molecular replacement, how do I extend the phase?
Further to Martyn's comment, we have a web page that addresses some of the
issues of using electron density as a model for Phaser, at
http://www-structmed.cimr.cam.ac.uk/phaser/density_as_model.html. It deals
specifically with the case of cutting out density from a crystallographic
map, but many of the considerations are the same for using EM density.
As for extending the phases, that's going to depend on whether or not you
have lots of non-crystallographic symmetry. If not, the EM model would
probably be most useful for finding substructures for experimental phasing.
Randy Read
