Re: [ccp4bb]: difficult crystal diffraction

[David W Borhani]

Paul, are you sure of your beam center? If not, try shifting (SFTOOLS?) the indices of the long axis +/- one, two, etc., orders. If you are misindexed (very easy on such a long cell), by having the beam center off by slightly over 1/2 the spot separation, you won't be able to scale.

Looking at the images, overlaps don't seem to be an issue, at least at low resolution (and you don't have hi-res data). Nice beam shape & resolution along the long axis.

Dave

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David W. Borhani, Ph.D.
Structural Biology Group Leader
Chemistry Department
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