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There seem to be systematic absences along l. Checking how those get indexed should tell you if the beam center is off, and if so by how much to correct it. Mounting in capillaries can be pretty brutal to crystals, especially if you are out of practice. Try Bob Thorne's system instead- loop the crystal as usual but instead of freezing it slip the pin into one of his polymer tubes with a bit of mother liquor in the end. Get the beamline people to bring out the old FTS temperature controller and set it for 2 degrees C, and you're all set! http://www.mitegen.com/products/micrort/micrort.shtml
Dear all, hopefully some of you may be able to make a few suggestions for a project I'm currently working on. It's a protein complex which crystallises to produce small crystals (max. dimension 0.1 mm, with rod morphology). It doesn't diffract in-house but does at synchrotron sources. The data is very anisotropic and a massive unit cell. I believe the indexing is P6 or P622 with cell dimensions 87.8, 87.8, 629.5. I've had a lot of problems scaling any data (I think, mainly due to overlaps or poor data and the anisotropy of diffraction). The crystals are frozen (but I do intend on trying some capillary mounted samples). Any suggestions regarding processing the data or optimising the data collection strategy would be wellcomed. If you would like to look at a typical frame collected at the ESRF on id23 at a distance of 445mm and a wavelength of 1.0 Ang. please feel free to download it from here: http://www.nottingham.ac.uk/~pazxtal Regards, Paul.. ################################## Dr Paul A. McEwan Post-Doctoral Research Fellow Protein Crystallography Group Centre for Biomolecular Science Clifton Boulevard University of Nottingham Nottingham NG7 2RD Tel: 0115 8468009 http://www.nottingham.ac.uk/~pazxtal ################################## This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
