I run ncont or distang - under Structure Analysis - Analyse Molecular contacts on the GUI. That will give you any contacts between domain 1 and 2, and the symmetry operator you need to apply to bring them together.
Say Domain 1 links to Domain 2 with sym opp -y,x-y,z [1, -1,0] you need to apply symm op 1-x, -1+x-y,z to domain 2 Use pdbset xyzin domain2.pdb xyzout domain2-s1.pdb symgen 1-x, -1+x-y,z end Then combine domain1 with domain2-s1.pdb to get the linked pdb Eleanor 劉家欣(OZ) wrote: > Dear All: > A crystal dataset was indexed as R3 space group and had 30% amino acid > sequence identity with the template. > Because of domain flexibility, split domains were separated to used to > find out the phase. > Fortunately, the phase was solved with two domains independently. > However, this two domains were far from each other. Is there any > program could combine them to one pdb? > Any suggestion would be appreciated. Thank you very much. > Best Regard, > jaishin
begin:vcard fn:Eleanor Dodson n:Dodson;Eleanor email;internet:[EMAIL PROTECTED] tel;work:+44 (0) 1904 328259 tel;fax:+44 (0) 1904 328266 tel;home:+44 (0) 1904 424449 version:2.1 end:vcard
