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Hello Ute, For removal of N-glycans: PNGaseF will cleave immediately at the asparagine. The enzyme sometimes needs slightly denaturing conditions (Triton X-100, SDS) for optimal efficiency, which, of course, might be problematic if you want to use the product in crystallization trials. For removal of O-glycans: O-Glycosidase will cleave between Gal and GalNAc. Success can be controlled by SDS-PAGE or preferably by MS. Oliver ------------------------------- Dr. Oliver H. Weiergraeber Institute for Neurosciences and Biophysics - Structural Biology - Research Centre Juelich D-52425 Juelich Germany Phone: +49-2461-612028 Fax: +49-2461-612020 ------------------------------- ----- Original Message ----- From: Ute Krengel <[EMAIL PROTECTED]> Date: Monday, July 10, 2006 6:08 pm Subject: [ccp4bb]: Deglycosylation tips wanted! > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Dear all, > > I am trying to get an overview over deglycosylation recipes. Any > good tips > about which enzymes to use and which procedures to follow and > controls to > perform are welcome! Also references are interesting to me. > > I promise to prepare a summary of the tips at the end! > > Cheers, > > Ute > > > > >
