Re: [ccp4bb]: Deglycosylation tips wanted!

[Ashley Buckle]

Hi Ute not a direct answer to your question, but you could also consider making mutants to avoid glycosylation.  If glycosylation sites are NXS/T then TA or SA mutation might help cheers ashley ----- Original Message ----- From: Ute Krengel <[EMAIL PROTECTED]> Date: Monday, July 10, 2006 6:08 pm Subject: [ccp4bb]: Deglycosylation tips wanted! ***  For details on how to be removed from this list visit the  *** ***          CCP4 home page http://www.ccp4.ac.uk         *** Dear all, I am trying to get an overview over deglycosylation recipes. Any  good tips about which enzymes to use and which procedures to follow and  controls to perform are welcome! Also references are interesting to me. I promise to prepare a summary of the tips at the end! Cheers, Ute Ashley Buckle Ph.D Senior Research Fellow The Department of Biochemistry and Molecular Biology School of Biomedical Sciences, Faculty of Medicine & Victorian Bioinformatics Consortium (VBC) Monash University, Clayton, Vic 3800 Australia http://www.med.monash.edu.au/biochem/staff/abuckle.html iChat/AIM: blindcaptaincat Tel: (613) 9905 3781 (office) Tel: (613) 9905 1653 (lab) Fax : (613) 9905 4699