Most likely result is that sites are wrong?
But check your correation coefficients between the anom signals from the
two data sets - maybe there is no real signal past a low resolution cut off?
Eleanor
[EMAIL PROTECTED] wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Hi: I am using Ccp4i (v 6.01) and started with a pair of SAD datasets (.sca
files from denzo) and then ran the shelxc/d/e suite with them. I got some
probably mediocre hits (cc<12%) but decided to use the best atoms from the
shelx hits and refine 'em with mlphare -- the sca file I described I ran
through scalepack,truncate and cad to get the mtz file to refine against the
shelx sites. I have made sure I'm refining anom.occupancy only (Se atoms) but
I find the sites and their occupancy are being consistently refined to 0,
often within ~8 cycles. Any ideas what I may be doing wrong?
Thanks for your help
Alex
begin:vcard
fn:Eleanor Dodson
n:Dodson;Eleanor
email;internet:[EMAIL PROTECTED]
tel;work:+44 (0) 1904 328259
tel;fax:+44 (0) 1904 328266
tel;home:+44 (0) 1904 424449
version:2.1
end:vcard
