[ccp4bb]: "negative" sequence numbers...

[Andrew Gulick]

I have a new structure in which I can see a residue N-terminal to the first methionine that is derived from the "left-over" gly-his that results after His-tag cleavage.  What is the proper way to label this residue (as far as sequence number) in order to have COOT and REFMAC properly handle the bond between the histidine and the "n-terminal" met?  (Should the residue number be 0?  -1?) I've tried a negative sequence label.  I've also tried to insert a residue with COOT (figuring it will build and label it correctly) and it "fails to find any acceptable configuration." making me wonder if it won't look residue N-terminal to residue #1. Thanks, Andy Andrew M. Gulick, Ph.D. ------------------------------------------------------ (716)  898-8619 Hauptman-Woodward Institute 700 Ellicott St  Buffalo, NY 14203 ------------------------------------------------------ Research Scientist Hauptman-Woodward Medical Research Institute Assistant Professor Dept. of Structural Biology, SUNY at Buffalo http://www.hwi.buffalo.edu/Faculty/Gulick/Gulick.html http://www.buffalo.edu/~amgulick