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I wonder how the crystals diffracted :-)
Something different I had a case during my PhD work where I could
decrease the mosaicity significantly from ~1.6 to 0.4 in average by a
very simple method. The same incubator was used 24x identical conditions
in two VDX plates sealed with silicon grease, one tray was directly
placed into the incubator and another one was floating on top of a
Styrofoam lid placed in 3 cm deep water. Guess which crystals had the
lower mosaicity. But I don't know why exactly this worked. The "regular"
trays are placed on heavy (maybe 20 kg) stone (marble?) plates to reduce
vibrations. Sometimes the fan starts to adjust the temperature but I
don't think this should have such a dramatic effect. I should also
mention that these crystals were more on the fast side of growing,
within 12 hours you would end up with something like 400x300x300
micrometers. One could actually see first tiny crystals shortly after
setting up the trays (manually, at that time this still took more than
10 minutes).
Anybody has an idea why this could have happened ?
Juergen
--
Jürgen Bosch
Howard Hughes Medical Institute and
University of Washington
Dept. of Biochemistry, K-418
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4542
FAX: +1-206-685-7002
