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I'm sure this has been posted before somewhere, but does anyone know how to deal with O-linked carbohydrates in CNS? Specifically, I have a protein with a fucosylated threonine. I noticed in the generate script there is a field for carbohydrate linkages, however the only one available for linkages to proteins is for N-linked sugars. Is there a quick way to deal with this, or am I stuck with having to generate topology/paramenter files for an entirely new residue consisting of Thr+fucose? Any advice would be great!

Thanks,

Eugene


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Eugene Hur, Ph.D.
Department of Biochemistry & Biophysics
University of California, San Francisco
MC 2240
600 16th St., GH-S416
San Francisco, CA 94158-2517
[EMAIL PROTECTED]




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