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pengpeng hao wrote:
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> Dear All
>
> Reccently I have a protein crystal having eight molecules in an asymmetric 
> unit. It is quite difficult to solve
> protein structure with molecular replacement method, so does anyone can tell 
> me some experiences about how to decrease 
> molecules in an asymmetric unit. The additive I used is Yttrium chloride! 
> Thanks in advance.
>
> Best Regards
>
> Hao
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>   
Dear Pengpeng,

It may be a good idea to look for the anomalous signal of Yttrium ions
and to solve the structure by SAD (single-wavelength anomalous dispersion).

Greetings

Wim Burmeister

-- 
*******************************************************************************
Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Laboratoire de Virologie Moleculaire et Structurale FRE 2854 CNRS-UJF
c/o EMBL, 6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9          FRANCE
E-mail: [EMAIL PROTECTED]
Tel:    +33 (0) 476 20 72 82       Fax: +33 (0) 476 20 94 00
http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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