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What makes you think you have eight molecules in the asymmetric unit if
you haven't solved the structure yet? You could try looking at the
self-rotation function too see if you can identify the NCS, and possibly
use it to help select from the molecular replacement solutions.

On Wed, 2006-08-30 at 02:09 +0000, pengpeng hao wrote:

> Reccently I have a protein crystal having eight molecules in an asymmetric 
> unit. It is quite difficult to solve
> protein structure with molecular replacement method, so does anyone can tell 
> me some experiences about how to decrease 
> molecules in an asymmetric unit. The additive I used is Yttrium chloride! 
> Thanks in advance.


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