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I am starting work on a protein that binds double-stranded DNA. In other dsDNA-protein stuctures I have noticed that some structures use blunt-ended DNA while others use sticky end DNA with 1,2 or 3 base G/C overhangs. Is there an advantage to using sticky-end vs. blunt-end DNA for crystallization? Also, what is the reason for using the G/C overhang?
