*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
In addition to the Tan paper that others have mentioned (which is extremely helpful), there a book chapter from Joachimiak and Sigler on this topic. Crystallization of protein-DNA complexes. Methods Enzymol. 1991;208:82-99. -- Kendall W. Nettles, PhD Assistant Professor Department of Biochemistry The Scripps Research Institute 5353 Parkside Dr. Jupiter Fl 33458 office 561-799-8851 fax 561-799-8957 cell 561-306-7566 > From: Ed Wright <[EMAIL PROTECTED]> > Date: Fri, 15 Sep 2006 08:39:07 -0400 > To: <[email protected]> > Subject: [ccp4bb]: DNA binding proteins > > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > I am starting work on a protein that binds double-stranded DNA. In other > dsDNA-protein stuctures I have noticed that some structures use blunt-ended > DNA while others use sticky end DNA with 1,2 or 3 base G/C overhangs. Is there > an advantage to using sticky-end vs. blunt-end DNA for crystallization? Also, > what is the reason for using the G/C overhang? >
