Could you have one indexing system for the native data and a different
one for the Se-MAD crystal?
Look at http://www.ccp4.ac.uk/dist/html/reindexing.html
For some SGs (P4i P3i H3 etc P2i3 ) this is possible.
If you run the Mege datasets in the experimental phasing module and
click on the
Automatically check and enforce consistent indexing button
The software will verify it for you.
Eleanor
Look at Liu Xin wrote:
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Dear all:
I've solved a structure by Se-MAD in solve,
and have converted the phase (solve.mtz) to cns (.hkl)
format in order to use cns refinement. I have
a native dataset with better resolution, when I include
both the native and phase data in cns and refine
the pdb built by resolve, R-factor stays at 55%.
If I use a model that I get from molecualr replacement
(use the resolve pdb as the research model, and research against
the native data), the R-factor goes down to 35%.
I was wondering why this happens? Is it appropriate to use
the molecular replacement model while still using the original phase
information (e.g. phase from solve)?
Thanks in advance for your help!
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