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Hi Pat,
I've worked with hollow rods in the past and could collect very good
data, even when the X-ray beam was shot through the middle including the
hollow void.
Good luck,
Dirk.
Patrick Loll wrote:
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We have a system where we can produce rod-shaped crystals that show a
classic depletion imperfection--the rods are essentially hollow for most
of their length, suggesting that growth is proceeding in this direction
more rapidly than fresh protein can diffuse into the area (picture a
long hexagonal rod with a perfect conical hole bored into one end,
extending for most of the rod's length).
Simple-minded optimization experiments (e.g., [PEG] vs. [salt] vs. pH)
have clear-cut effects on nucleation, but don't seem to alter crystal
shape, which I interpret as meaning that growth occurs rapidly once
nucleation has taken place.
What are peoples' thoughts on improving the morphology? We're currently
exploring whether additives can selectively poison growth along the long
axis, but I'd be curious to hear other ideas. For example, might gels
help, or are they mainly effective in controlling nucleation?
They ARE pretty cool looking. I wonder if there's a market for
nanovases...
Pat
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Patrick J. Loll, Ph. D. (215) 762-7706
Associate Professor FAX: (215) 762-4452
Department of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
[EMAIL PROTECTED]
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Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: [EMAIL PROTECTED]
http://sb.web.psi.ch
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