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Dear Nick
one year ago I tested several crystallization robots and
in particular the Honeybee 961 from Cartesian.
We were rather disappointed by the performances of the
machine. for several reasons:
-the dispensing part of the protein is really a poor
design. it is prone to clogging.
-the 96-well head is quite a sensitive piece of work and
if the plate is misplaced you will collapse and crash all
96 needles against the surface of the plate at once. you
can imagine what is to follow after this kind of
"accident"
-the machine is very "thirsty", it need gallons or liters
of water to clean because there is no disposable part so
if you want to avoid cross contamination with proteases or
rnases (we handle RNAs) this can be a concern especially
if you have multiple users.
Now if you are looking for a very reliable, clean and
user-resistant and user-friendly machine, I would
recommend you consider the Mosquito from TTP Labetch (they
are based in UK). We bought one one year ago and more than
1o labs at UCSF use it successfully with all kind of
targets: proteins, RNAs and membrane proteins. it works
very well in hanging drops and sitting drops.
Hope this will help.
Best
Pascal Egea
University of California San Francisco
Department of Biophysics and Biochemistry
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Hello,
Does anyone have any experiences (good or bad) of using
the Cartesian Honeybee 961 robot for setting up
crystallisation experiments? I'm particularly interested
in the reliability of the 96-well head that dispenses the
precipitants.
Thanks very much,
Nick
----------------------
NM Burton, Biochemistry
[EMAIL PROTECTED]
