On Wed, 2007-02-14 at 07:59 -0800, mac minista wrote: > Dear all, > > I have noticed that going from refmac 5 (script) to generate fo-fc and > 2fo-fc maps in O / Pymol once and Coot in the other, the outcome is > not exactly the same electron density maps-I mean some differences are > seen. I have used the following columns in Coot (latest version): > FOFCWT, PHFOFCWT and 2FOFCWT, PH2FOFCWT. The .o map file was > generated using FFT, MAPMASK and MAPMAN. > I want to know why I am not getting identical maps, and if there is a > solution to avoid this problem then I would really appreciate your > help.
Well, the devil is in the detail. a MAPMAN map in O vs a REFMAC MTZ in Coot. Somewhat apples and oranges. Stating the obvious: the griding and contour level may well be different. The contouring algorithms certainly are. How about a Coot/Refmac + Mapman map in O or a MAPMAN map in Coot? Then the differences will be more clear? What do you get if you overlapmap ADD 1 -1 and look at differences there?
