-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hi Andrew,
Can you get cDNA from human cell lines? Perhaps one of your colleagues can provide you with a few microliters. You can amplify from there all the genes you need (provided they are transcribed in that cell line; therefore, better use cDNA from two-three cell lines) We have followed this approach quite successfully. Cheers, Miguel Andrew Wong escribió: > Sorry a little offtopic... > > We'r trying to clones a number of putative human proteins for > crystallization. Besides IMAGE clones from OpenBiosystem, is there any > the cheaper way of obtaining human cDNA clones? OpenBiosystem is okish, > ~$100Cdn for each clone, but does get abit expensive when you start > getting alot. > - -- Miguel Ortiz Lombardía Centro de Investigaciones Oncológicas C/ Melchor Fernández Almagro, 3 28029 Madrid, Spain Tel. +34 912 246 900 Fax. +34 912 246 976 email: [EMAIL PROTECTED] www: http://www.ysbl.york.ac.uk/~mol/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Le travail est ce que l'homme a trouvé de mieux pour ne rien faire de sa vie. (Raoul Vaneigem) -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.2.2 (GNU/Linux) iD8DBQFF2r9hF6oOrDvhbQIRAp1KAKCb7MKwkjtH7Nn+Jw0RyO6IPJmKdwCfbu3S opLlcpYxP7uzicyX0B6U4aY= =NH4Q -----END PGP SIGNATURE-----
