Forgot to mention that the NCSs are improper.
On Feb 21, 2007, at 6:22 PM, Jianghai Zhu wrote:
Dear all,
I have two low resolution (3.8 A) MAD data sets from two different
space groups. There are 4 copies of my molecule in one space group
and 2 copies in the other. The density-modified maps from these
data sets are poor, but still allow me to build a crude model on
them. All the domains should be at the right or close to right
orientations and positions. I would like to try multi-crystal
averaging to improve my maps. Since I already have the model, I
used NCSMASK to make 6 masks for the 6 copies of my model. Then I
used DMMULTI to perform the multi-crystal averaging. The maps came
out of DMMULTI showed great improvement. Some densities were not
seen before showed up nicely. However, after I read the manual
carefully, I realize that DMMULTI would not take care of the mask
overlap. So some regions in my density map between the NCS-related
and symmetry-related molecules must be messed up. Could any
expert out there give me some suggestions about how to make proper
NCS masks for DMMULTI? Thanks at advance.
Jianghai
