You just need one mask for your master molecule, which will be
transferred by the matrices you give to all the other copies.
I make the mask from the crude model, with a generous border round it -
the program will also prune it to get rid of overlaps..
Eleanor
Jianghai Zhu wrote:
Dear all,
I have two low resolution (3.8 A) MAD data sets from two different
space groups. There are 4 copies of my molecule in one space group
and 2 copies in the other. The density-modified maps from these data
sets are poor, but still allow me to build a crude model on them. All
the domains should be at the right or close to right orientations and
positions. I would like to try multi-crystal averaging to improve my
maps. Since I already have the model, I used NCSMASK to make 6 masks
for the 6 copies of my model. Then I used DMMULTI to perform the
multi-crystal averaging. The maps came out of DMMULTI showed great
improvement. Some densities were not seen before showed up nicely.
However, after I read the manual carefully, I realize that DMMULTI
would not take care of the mask overlap. So some regions in my
density map between the NCS-related and symmetry-related molecules
must be messed up. Could any expert out there give me some
suggestions about how to make proper NCS masks for DMMULTI? Thanks at
advance.
Jianghai
