Ronaldo I have a database of crystallization conditions extracted from the PDB. I was able to parse the data to extract the concentration of protein in around 900 cases.
The lowest 9 protein concentrations were as follows: PDB ID TYPE DATE Protein conc, mg/ml 1EYM ISOMERASE. 07/05/2000 0.75 1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1 1W5G FOUR HELIX BUNDLE. 06/08/2004 1 1UU4 HYDROLASE. 15/12/2003 1 1W2U HYDROLASE. 08/07/2004 1 1DXP SERINE PROTEASE. 13/01/2000 1 1DY8 SERINE PROTEASE. 18/01/2000 1 1DY9 SERINE PROTEASE. 31/01/2000 1 1DG6 APOPTOSIS. 23/11/1999 1.2 The maximum protein conc was 200 mg/ml (1BLF) My database goes up to October 2004. Janet Newman and Tom Peat have done this job far more conscientiously and could probably give you better and more up-to-date data. Patrick On 2/23/07, Ronaldo Alves Pinto Nagem <[EMAIL PROTECTED]> wrote:
Dear all, As protein crystallization is the main topic of the day may I include another question? What was the minimun protein concentration reported with success in crystallization trials? I ask that because the protein I am trying to crystallize is much less soluble than the one mentioned in the lasts emails. Thanks Ronaldo.
