PreScission protease worked great for all proteins and constructs I tested and purified. The cleavage is very specific and works in a decent range of salt conditions as well.
Sometimes, you may have to optimize digestion time and enzyme/protein ratio to get 100% or maximum-possible cleavage. Raji ---------Included Message---------- >Hi, > >A non-ccp4 Q. Sorry. > >I would like to use a cleavable purification tag at the N-terminus/ >extracellular end of my membrane protein for purification. Before I >start, I wonder if someone could recommend a particular protease site >that I can engineer between the tag and my protein? How about a >proprietary cleavage system such as the PreScission protease (GE >Healthcare)? I would be grateful to hear success and horror stories >in this area. > >Best wishes, > >Rene > >================================================ >Dr R.A.W. Frank, PhD >Royal Commission for the Exhibition of 1851 Research Fellow > >Prof Seth Grant Lab / Genes to Cognition >Wellcome Trust Sanger Institute >Hinxton >Cambridge CB10 1SA > >Work Tel: 0044 (0)1223 834244 ext. 7318 >Cell No.: 0044 (0)7870 208280 >=============================================== > > > > > ---------End of Included Message----------
