You dont say the spacegroup. Different MR solutions can exist relative
to any of the acceptable alternate origins.
Eg If it were P21 say the two solutions could lie anywhere along the b axis.
Try running superpose (coordinate utility ) matching sequences. If the
rotation looks like a symmetry operator then that is probably what has
happened..
Re the refinement - that is another more difficult problem at 3A with
37% sequence ID...
Eleanor
thunderbird wrote:
Hi all.
I am trying to solve a structure with one molecules in the asu through MR.
Using a not good data set at 3.0A and a structure with sequence similarity of
37%, Phaser gave a result of z score 14.7 and LL-gain 220, probably a right
solution.
But when I slightly modified the model I got from Phaser and used it as an
input to run Phaser again, I got another solution overlapped partly with the
first solution. I don't know how it could happen like that. And the Rfree
factor could not go
below 0.43 in the following refinemet. I am wondering what is wrong with it?
thanx in advance
Lei Yin
Lei Yin, Ph.D.
National Laboratory of Biomacromolecules
Institute of Biophysics
Chinese Academy of Sciences
No. 15 Datun Road, Beijing 100101, China
E-mail:[EMAIL PROTECTED]
Tel: 86-10-64888548
