Dear All, We have solved a structure that has an intersubunit His-His H-bond (ND1---NE2). The protein most likely undergoes conformational changes that involve the making and breaking of H-bonds at this interface. In all the protein structures I have previously studied I don't recall ever seeing such a bond - His-His ring stacking yes, but never H-bonding. I was wondering if anyone else had seen this type of interaction and whether it had any functional significance. The crystals were grown at pH 5.6.
Many thanks Dave Lawson ------------------------------- Dr. David M. Lawson Biological Chemistry Dept., John Innes Centre, Norwich, NR4 7UH, UK. Tel: +44-(0)1603-450725 Fax: +44-(0)1603-450018 Email: [EMAIL PROTECTED] Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm
