This is very protein-specific, for some proteins it is better to co-crystallize with an inhibitor, then countersoak with a different inhibitor, yet for others it is better to co-crystallize with an inhibitor of interest directly. For all it's worth, I personally am a proponent of the second approach, since soaking can and does generate scary artifacts.
Artem > Hi, all, > I met some crystal structures with disordered active sites. Soaking > common ligands can not make it become ordered. I am wondering what > people generally do in such situation. > > Thanks, > > Nian Huang >
