Hi Michal, Hmmm... Was it kicked out upon crystallisation or was crystallisation possible because most of your pentamers were not fully occupied by ligand so allowing the possibility of good contacts? ;-)
What was the excess of ligand? Do you know its affinity? Miguel 2007/6/24, Michal Harel <[EMAIL PROTECTED]>:
Hi all, We have a structure of a pentamer which was crystallized from a complex of the protein with a ligand. The structure shows that 4 out of 5 binding sites are occupied. The symmetry-related molecules pack much tighter around the unoccupied monomer. I am looking for examples of structures in which a ligand was "kicked out" of a binding site upon crystallization. Regards - Michal. ------------------------------------------------------------------------ Dr. Michal Harel Dept. of Structural Biology [EMAIL PROTECTED] Weizmann Inst. of Science tel: 972-8-9342647 Rehovot 76100, ISRAEL fax: 972-8-9344159 ------------------------------------------------------------------------
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