Dear CCP4 message board,
I am having a problem with electron density that looks as if it has been
compressed along the Y axis. Density that would appear as a sphere looks
like an ellipsoid, and the effect seems worse in some regions (typically
where I am having problems building in a flexible loop). It is worse in
my Fo-Fc map (compared to my 2Fo-Fc) which makes me wonder if there is a
problem with my calculated F's?
Does anyone know of common reasons for seeing this effect, and how to
fix it?
(I processed the data in Mosflm, scaled in Scala, phased in phaser and
then used the usf program dataman to pick my R free set and convert to
cns format, and have been using anneal, minimize and model_map in CNS).
Simon
