Das, Debanu wrote:
Hi,
There are at least 4 methods to try to estimate amount of detergent in a membrane protein crystal
.............
In summary, someone wanting to estimate amount of detergent in their
crystals and have sufficiently large and numerous crystals, could try out
any of the above methods. The above techniques are quite well documented
in literature. The first 3 can be done in-house and so can FTIR.
I happen to have the reference for Ron Kaplan's TLC method at my fingertips:
Eriks, L. R., Mayor, J. A. & Kaplan, R. S. (2003). A strategy for
identification and quantification of detergents frequently used
in the purification of membrane proteins. Anal Biochem 323, 234-41.
Sensitivity is limited by the amount of aqueous solution you can spot on a TLC
plate.
Probably could improve sensitivity by speed-vac-ing an aliquot to near dryness
and redissolving in 50% methanol or something.
