Can you show the map? Anthony
> Dear all > > I am refining a peptide structure with an N-terminal throenine. > However, the density map of the N-terminal part looks strange, > the first throenine does not fit in well while the second valine > fits very well. It seems that this throenine was modified somehow > during protein production. The peptide was expressed in E.Coli > and cleavaged by Formic Acid and Cyanogen Bromide, then > purified by HPLC and lyophilized. > > Does anyone have similar experience of a modified N-terminal > throenine? If so, please kindly point to me a reference. > > Thank you. > > Jie Liu >
