S/T formylation by formic acid can be extensive and
should probably be expected.
steve
On Fri, 28 Sep 2007, [EMAIL PROTECTED] wrote:
Can you show the map?
Anthony
Dear all
I am refining a peptide structure with an N-terminal throenine.
However, the density map of the N-terminal part looks strange,
the first throenine does not fit in well while the second valine
fits very well. It seems that this throenine was modified somehow
during protein production. The peptide was expressed in E.Coli
and cleavaged by Formic Acid and Cyanogen Bromide, then
purified by HPLC and lyophilized.
Does anyone have similar experience of a modified N-terminal
throenine? If so, please kindly point to me a reference.
Thank you.
Jie Liu
--
Steve Kaiser
Kopito Lab
Stanford University
Lokey Laboratories, Room 154
337 Campus Loop Drive
Stanford CA, 94305
tel:650-723-7588
fax:650-723-0155
mail: [EMAIL PROTECTED]
