Hi Jan, On Tue, Nov 20, 2007 at 11:19:24AM -0800, Jan Abendroth wrote: > Hi all, > I am trying to include exteral phase information in a sharp refinement. The > phases come from dmmulti. > Steps this far: > - convert PHI and FOM to HLA and HLB in sftools, > - rename PA and PB to HLA and HLB in cad > - copy file (xx.extern.mtz) with all F, DANOs and HLA HLB into my datafiles > directory > - make sure that I am an "expert user" in Sharp > ... now, how can I tell Sharp to use the external phases? Is it happy with > HLA/HLB only?
No - SHARP needs all (HLA, HLB, HLC and HLD). Unfortunately (as you discovered), DMMULTI doesn't write them as opposed to DM. To convert those into HLA-D I woudl recommend using chltofom (from Kevin Cowtans Clipper tools): it is available through the CCP4i. > Once we are at it - how can one define in Sharp for a MIRAS case a native > reference data set for which no anomalous data were collected. Sharp seems > to need a DANO column. You don't need to give it the DANO/SANO/ISYM columns - just leave them at default 'None'. Cheers Clemens > > Thanks a lot > Jan -- *************************************************************** * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * * Global Phasing Ltd. * Sheraton House, Castle Park * Cambridge CB3 0AX, UK *-------------------------------------------------------------- * BUSTER Development Group (http://www.globalphasing.com) ***************************************************************
