Richard Gillilan wrote:
I am currently working on guidelines for when helium and microbeam
are necessary (based on both simulations and explicit measurements).
At the present time, my feeling is that crystals below 50 micron can
certainly make the extra hassle worthwhile. It really depends upon
how badly you want that extra resolution. In the case above, it
pushed the resolution from above to below the 2.0 Angstrom mark based
on I/SIG.
Hi Richard,
I/SigI based on which program ? Default modes or tweaked by expert ? I
would give this particular dataset a chance to be processed by all
available programs and then do the comparison, or actualy all the
datasets you have with various setups. Should be a nice table comparing
program X versus Y and Z with the given data. And if it's SeMet data
that would even be better - runnig e.g. Shelx and demonstrating which
setup leads to an interpretable electron density.
Juergen
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
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Web: http://faculty.washington.edu/jbosch