Directed Evolution Approaches in Structural Biology
Wed 30th & Thu 31st January 2008 at EMBL Grenoble, France.
Funded by: SPINE2COMPLEXES & TEACH-SG (EU FP6)
Info & Register: www-db.embl.de/jss/EmblGroupsOrg/conf_91
See also: www.spine2.eu/SPINE2/meetings/index.jsp?m=28
A number of laboratories are now developing or using library-based
strategies in combination with structural biology. This may be to
overcome problems in the structure solution process (expression,
crystallisation etc.) or to study/engineer proteins for structural and
functional studies. We aim to strengthen links between these labs and
provide training for scientists interested in this field.
Topics
1. Library strategies for “difficult-to-express” proteins
2. Scaffold proteins and binders as cocrystallisation chaperones
3. Combinatorial approaches to protein complex definition and expression
4. Structural studies of in vitro evolved proteins
·Colony filtration (CoFi).
·Combinatorial Domain Hunting (CDH).
·Efficient fragmentation, truncation & mutation approaches
·Robotics & truncation libraries.
·GFP solubility screening.
·Screening by FACS and fluorescent colony picking.
·DARPins & ribosome display.
·DNA shuffling/Oil-in-water emulsions.
·Use of display technologies
Speakers
Stephanie Cabantous Waldo Lab, Los Alamos, USA
Thomas Huber Plückthun Lab, University of Zurich
Speaker Nordlund Lab, Karolinska Institute, Sweden
Renos Savva Domainex Ltd, UK
Darren Hart EMBL Grenoble, France
Martin Walsh MRC France, Grenoble
Amir Aharoni Weizmann Institute, Israel
Sabine Mazaleyrat AstraZeneca (ex), UK
Chris Meier UCB-Celltech, UK
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Dr. Darren Hart,
Team Leader
High Throughput Group
Grenoble Outstation
European Molecular Biology Laboratory (EMBL)
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http://www.embl.fr/groups/htt/expression.html
Email: [EMAIL PROTECTED]
Tel: (33) 4 76 20 77 68
Fax: (33) 4 76 20 71 99
Postal address: EMBL, 6 rue Jules Horowitz, BP181, 38042 Grenoble, Cedex
9, France
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