we have recently tried the new lid mentioned below. It works very well, Mosquito makes 3 hanging drops 96 well plate in 3-4 minutes! The film is very easy to handle and it is very transparent. The price for the lid is significantly reduced: from 13€ before to 4€ now.
Comparing to Cartesian and Phoenix robots the Mosquito is REALLY maintenance free machine, especially the third generation insect where many 1st and 2nd Mosquito generation users advices have been implicated. Alexey Rak Structural Biology, Chemical Sciences Sanofi-Aventis Centre de Recherche Paris -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Shirley Roberts Sent: Friday, January 11, 2008 7:25 PM To: [email protected] Subject: Re: [ccp4bb] crystallisation robot I sent this personal reply on wednesday to Madhavi Nalam's original question. In the light of recent comments I'm posting it to the BB to support TTP labtech and Mosquito! Some of the points have been made by others now, excuse the repeats! We at YSBL have been very happy with our mosquito. We use it to setup sitting drops with the MRC Wilden plate, this can be for screening or optimisation (not always necessary to go to 24-well format). You can also setup hanging drops, this can work better if detergents are involved. A new lid has just been released from TTP that costs £2.50 and takes 3 drops per well but we havn't tried this yet. You will need another robot (or multichannel pipette) for transfering the screen solutions.We use the hydra from alpha-helix ltd with flexible needles so hard to break. The phoenix robot does both jobs and seems popular. The Douglas robot has been mentioned, the Cartesian is the other main robot in use but I don't have any personal experience of it, I've heard it is quite high maintenance. Good luck with your purchase! Shirley Roberts
